Clickable Cubosomes for Antibody-Free Drug Targeting and Imaging Applications

N Alcaraz; Q Liu; E Hanssen; A Johnston; BJ Boyd

Journal article

Clickable Cubosomes for Antibody-Free Drug Targeting and Imaging Applications

N Alcaraz, Q Liu, E Hanssen, A Johnston, BJ Boyd

Bioconjugate Chemistry | AMER CHEMICAL SOC | Published : 2018

DOI: 10.1021/acs.bioconjchem.7b00659

Abstract

The combination of copper-free click chemistry with metabolic labeling offers new opportunities in drug delivery. The objective of this study was to determine whether cubosomes functionalized with azide or dibenzocyclooctyne (DBCO) groups are able to undergo copper-free click chemistry with a strained cyclooctyne or azide, respectively. Phytantriol-based cubosomes were functionalized using phospholipids bearing an azide or DBCO group. The modified cubosome dispersions were characterized using dynamic light scattering, cryo-TEM, and small-angle X-ray scattering. The efficiency of "clickability" was assessed by reacting the cubosomes with a complementary dye and determining bound and unbound d..

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University of Melbourne Researchers

Eric Hanssen Author

Related Projects (1)

ARC CENTRE OF EXCELLENCE IN CONVERGENT BIO–NANO SCIENCE AND TECHNOLOGY

The CoE in Convergent Bio-Nano Science &Technology comprises a multi-disciplinary team focused on research aiming to understand and control ..

Grants

Awarded by Australian Research Council

Funding Acknowledgements

This research was undertaken on the SAXS/WAXS beamline at the Australian Synchrotron, Victoria. This work was partly funded by the Australian Research Council under the ARC Discovery Projects scheme and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology.